How to Prepare a 1mM Solution of Silver Nitrate AgNO3

Preparation of a 1mM Silver Nitrate (AgNO3) solution is a crucial step in various chemical and pharmaceutical experiments. This article will guide you through the process of preparing this solution accurately, ensuring you have the correct parameters in place.

Materials Needed

Silver nitrate (AgNO3) solid Distilled water A balance for precise weighing A volumetric flask or a graduated cylinder A stirrer or a magnetic stirrer to ensure the solution is well mixed

Steps to Prepare the Solution

Calculate the Amount of AgNO3 Required

To prepare 1 liter (1000 mL) of a 1mM solution, follow the formula below:

Amount (g) Molarity (mol/L) times; Volume (L) times; Molar Mass (g/mol)

Giving us: Amount (g) 0.001 mol/L times; 1 L times; 169.87 g/mol ≈ 0.16987 g

Accurately Weigh the Silver Nitrate

Use a balance to weigh approximately 0.16987 grams of AgNO3.

Dissolve the AgNO3 in Water

Add the weighed AgNO3 to either a volumetric flask or a beaker. Dissolve the solid in a small volume of distilled water (100-200 mL) and stir until the solid is completely dissolved.

Make Up the Volume to 1 Liter

Transfer the solution to a 1 liter volumetric flask if necessary. Add distilled water until the total volume reaches 1 liter.

Mix the Solution

Ensure the solution is well mixed. This can be done by inverting the flask a few times or using a stirrer.

Important Notes

Label the solution with the concentration and date of preparation. Store the solution in a dark container. Silver nitrate can decompose upon exposure to light.

This method ensures the preparation of a 1mM solution of silver nitrate, which is widely used in numerous scientific applications and experiments.

Additional Information on Dilution Calculations

For different volumes, the amount of silver nitrate required can be calculated using the formula:

Amount (g) Molarity (mol/L) times; Volume (L) times; Molar Mass (g/mol)

Example:

To prepare 50 mL of a 1mM solution:

Amount (g) 0.001 mol/L times; 0.05 L times; 169.87 g/mol ≈ 0.0084935 g ≈ 8.5 mg

In practical terms, this means:

1mM Silver Nitrate solution: Add 0.1699 g of AgNO3 to 1 liter distilled water.

50 mL of 1mM AgNO3 solution: Weigh out 8.5 mg of AgNO3, dissolve it in a small volume of water, and make up the final volume to 50 mL in a standard volumetric flask.

Usage Example

In a study involving Tulsi Ocimum sanctum leaf extract, 50 mL of 1mM AgNO3 solution was prepared by adding 0.1699 g of AgNO3 to 1 liter of distilled water. This solution was then used in an experiment where 300 mL of the freshly prepared 1mM AgNO3 solution was added to a 50 mL Tulsi Ocimum sanctum leaf extract in a 500 mL conical flask. The flask was incubated in the dark for 24 hours at room temperature.